Flow Forward Bring your scRNA seq analysis to light in an. Accelerate your discovery with the leading platform for single-cell flow cytometry analysis. We’re here to help you accelerate routine phenotyping, take your immunology research to the next level, and get you from data to resultsone cell at a time. Welch’s t test was used for statistical comparison between HD NK from freshly isolated PBMC and HD expanded NK cell groups and separately between expanded HD and BCP NK cells. FlowJo is your biggest fan and strives to be an outstanding source of support. Pecent-positive expression of the markers was then tabulated and graphed ( n = 3–8, mean ± SEM).
Cells were gated on a CD45 +CD56 +CD3 – population and then analysed using FlowJo Software for the expression of other markers. c Freshly isolated PBMCs from healthy donors ( HD), expanded HD NK cells, or expanded breast cancer patient ( BCP) NK cells were stained for the expression of CD45, CD56, CD3, CD16, NKG2D, NKp44, NKp46, NKp30, CD69, CD160, CD11b, CD27, CD25, NKG2A, CD158a, CD158b, and CD158e1. Unpaired Student’s t test was used to compare the difference between groups. Fold expansion was calculated assuming that NK cells comprise 15% of PBMCs ( n = 3, mean ± SEM). Cells were counted every week using trypan blue. Please check your Spam or Junk folder if you do not receive the email within 15 minutes.
Flowjo 10 frozen serial number#
You will be emailed a trial serial number to put into the software. Fill out the form below using your hardware ID. Get your computer's hardware ID ( instructions here ). Antibodies (2 g per sample) were incubated with 10 l Protein A Dynabeads for two hours on a rotator at 40 rpm at 6C. For each sample, ChIP assays were performed in parallel with antibodies directed against H3K27ac (C15410196, Diagenode) and H3K4me3 (C15410003, Diagenode).
Flowjo 10 frozen install#
Co-culture was supplemented with IL-2 and new media every 2–3 days, and with irradiated feeder cells on a weekly basis. Download and install the latest version of FlowJo. Before immunoprecipitation, a 10 input sample was removed. b Frozen peripheral blood mononuclear cells ( PBMCs) from breast cancer patients ( red) or healthy donors ( blue) were cultured with irradiated feeder cells K562mbIL21 and 100 U/μL of IL-2. Frozen 2 Disney Deluxe Figurine Playset Action Figures 10 Piece Figure Set. a A schematic showing the expansion protocol of NK cells. Expansion and phenotype of expanded natural killer ( NK) cells from breast cancer patients and healthy donors.
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